Biacore is based on the surface plasmon resonance (SPR) technology. It features real-time detection, label-free operation, excellent sensitivity, and reliable data quality. It can detect a wide variety of molecular types, including almost all biomolecules such as small molecule compounds, proteins, nucleic acids, polysaccharides, etc.

It can be applied in the following aspects:

1. Kinetic analysis: Precisely determine the association rate constant (ka) and dissociation rate constant (kd) of biomolecular interactions, and thus calculate the affinity constant (KD), providing key data for a deep understanding of the molecular interaction mechanism.

2. Affinity determination: Accurately evaluate the strength of affinity between different biomolecular pairs, helping to screen high-affinity molecular pairs, which is of great significance in drug target discovery and optimization.

3. Concentration determination: By comparing with reference samples of known concentrations, the concentration of biomolecules in unknown samples can be quantitatively analyzed.

4. Specificity analysis: It can effectively distinguish specific binding from non-specific binding, determine the specificity of biomolecular interactions, and provide strong support for the study of complex biological systems.

Training time and location: March 20, 2025 (Thursday), A302

Registration method: Please register as a unit of the research group to lqye@siii.cas.cn. Due to venue limitations, training will be grouped based on the number of applicants.

Registration deadline: 17:00, March 20, 2025.

Contact: YE Linqian, 021-54923034

Institutional Center for Shared Technologies and Facilities

Analytical Core Facility