Academic Experience:

2024-present, Associate professor, Shanghai Institute of Immunity and Infection, CAS 

2021-2024, Postdoctoral fellow, Shanghai Institute of Immunity and Infection, CAS

Education:

2013-2020, Ph.D., Huazhong Agricultural University (HZAU)

2008-2013, B.S., Huazhong Agricultural University (HZAU)

Our laboratory focuses on important bacterial pathogens, such as Salmonella and Klebsiella. By developing and utilizing novel high-throughput RNA sequencing approaches, we aim to globally profile the RNA transcriptomes and interactomes of bacteria under various physiological and pathological conditions, and identify novel non-coding small RNAs and their regulatory functions during bacterial infections. These studies will further deepen our understanding of the molecular mechanisms of gene expression regulation in host-bacteria interactions, and provide new strategies for the development of new antibacterial drugs and vaccines.

Selected publications

1. Jing Zhang^#, Shuo Zhang^#, Wei Zhou^#, Xiang Zhang^#, Guanjin Li, Ruoxuan Li, Xingyu Lin, Ziying Chen, Fang Liu, Pan Shen, Xiaogen Zhou, Yue Gao, Zhenguo Chen, Yanjie Chao, Chengyuan. A widely conserved protein Rof inhibits transcription termination factor Rho and promotes Salmonella virulence program. Nat Commun. 2024 Apr 15;15(1):3187.

2. Fang Liu^#, Ziying Chen^#, Shuo Zhang, Kejing Wu, Cheng Bei, Chuan Wang, Yanjie Chao. In vivo RNA interactome profiling reveals 3'UTR-processed small RNA targeting a central regulatory hub. Nat Commun. 2023 Dec 7;14(1):8106.

3. Fang Liu, Yongfang Huang, Changxian Wu, Longchuan Duan, Huanchun Chen, Zhengfei Liu. Genome-wide transcription start site mapping in the facultative intracellular pathogen Brucella melitensis by Capping-seq. Gene. 2022 Nov 30;844:146827.

4. Fang Liu, Ke Zheng, Huanchun Chen, Zhengfei Liu. Capping-RACE: a simple, accurate, and sensitive 5′ RACE method for use in prokaryotes. Nucleic acids research, 2018, 46(21): e129-e129.

5. Ke Zheng, Yang Wang, Na Li, Fangfang Jiang, Changxian Wu, Fang Liu, Huanchun Chen, Zhengfei Liu. Highly efficient base editing in bacteria using a Cas9-cytidine deaminase fusion. Communications biology, 2018, 1(1): 1-6.

6. Changxian Wu^#, Xianjin Xu^#, Ke Zheng, Fang Liu, Xudong Yang, Chuangfu Chen, Huanchun Chen, Zhengfei Liu. Characterization of ribonuclease III from Brucella. Gene, 2016, 579(2): 183-192.